SEMA4F Antibody - N-terminal region
Catalog Number:
ASB-ARP46421_P050
Product Overview
Article Name:
SEMA4F Antibody - N-terminal region
Biozol Catalog Number:
ASB-ARP46421_P050
Supplier Catalog Number:
ARP46421_P050
Alternative Catalog Number:
ASB-ARP46421_P050-25UL,ASB-ARP46421_P050-100UL
Manufacturer:
Aviva
Host:
Rabbit
Category:
Proteine/Peptide
Application:
WB
Species Reactivity:
Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen:
The immunogen is a synthetic peptide directed towards the n terminal region of human SEMA4F
Alternative Names:
S4F, SEMAM, SEMAW, M-SEMA, PRO2353, m-Sema-M
Product Description
SEMA4F has growth cone collapse activity against retinal ganglion-cell axons.
Product Properties
Clonality:
Polyclonal
Concentration:
0.5 mg/ml
Molecular Weight:
67kDa
NCBI:
10505
UniProt:
O95754-2
Form:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Images
human cell line A431 Primary antibody dilution and incubation time:1:600, 4 degree overnightSecondary antibody used and dilution and incubation time: 1:3000, RT 2 hours
WB Suggested Anti-SEMA4F Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T
Lanes: 1. Untransfected MIA-PACA2 cell lysate 2. Untransfected MIA-PACA2 cell lysate 3. Untransfected MIA-PACA2 cell lysate 4. hSEMA4F transfected MIA-PACA2 cell lysate 5. Untransfected MIA-PACA2 cell lysate 6. hSEMA4F transfected MIA-PACA2 cell lysate Primary Antibody Dilution: 1:600 Secondary Antibody: Donkey Anti-rabbit HRP Secondary Antibody Dilution: 1:3000 Gene Name: SEMA4F Submitted by: Dr. Tianliang Sun, Max Planck Institute for Heart and Lung Research
Lanes: 1. A431 cell lysate + control siRNA 2. A431 cell lysate + SEMA4F siRNA Primary Antibody Dilution: 1:600 Secondary Antibody: Donkey Anti-rabbit HRP Secondary Antibody Dilution: 1:3000 Gene Name: SEMA4F Submitted by: Dr. Tianliang Sun, Max Planck Institute for Heart and Lung Research
SEMA4F antibody - N-terminal region (ARP46421_P050) in human A431 using Western Blot