COX15 Antibody - N-terminal region

Product Overview

• Article Name: COX15 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP46442_T100
• Supplier Catalog Number: ARP46442_T100
• Alternative Catalog Number: ASB-ARP46442_T100-25UL,ASB-ARP46442_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human COX15
• Alternative Names: MC4DN6, CEMCOX2

Product Description

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane.Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates several transcript variants diverging in the 3 region including alternate poly A sites. In total, 2 different isoforms are encoded by these variants.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 46 kDa
• NCBI: 1355
• UniProt: Q7KZN9-2
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-COX15 Antibody
Catalog Number: ARP46442
Paraffin Embedded Tissue: Human Muscle
Cellular Data: Skeletal muscle cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X - See more at: http://www.avivasysbio.com/cox15-antibody-n-terminal-region-arp46442-t100.htmlsthash.41yjSrAu.dpuf

WB Suggested Anti-COX15 Antibody Titration: 5.0ug/ml
Positive Control: HepG2 cell lysateCOX15 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells

Lanes:
Lane1: 20ug human fibroblast mitochondria
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
COX15
Submitted by:
Oleh Khalimonchuk, University of Nebraska-Lincoln

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

COX15 antibody - N-terminal region (ARP46442_T100) in Human HepG2 using Western Blot