ACSL3 Antibody - N-terminal region

Product Overview

• Article Name: ACSL3 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP46453_P050
• Supplier Catalog Number: ARP46453_P050
• Alternative Catalog Number: ASB-ARP46453_P050-25UL,ASB-ARP46453_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACSL3
• Alternative Names: ACS3, FACL3, LACS3, LACS 3, PRO2194

Product Description

ACSL3 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 80 kDa
• NCBI: 2181
• UniProt: O95573
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment.

Researcher: Received from anonymous
Application: IHC
Species+tissue/cell type:THP-1 derived macrophage
Primary antibody dilution: 1:200
Secondary antibody: Goat anti-rabbit Alexa Fluor 647
Secondary antibody dilution:1:333

Researcher: Received from anonymous
Application: IHC
Species+tissue/cell type:THP-1 derived macrophage activated with iMtb
Primary antibody dilution: 1:200
Secondary antibody: Goat anti-rabbit Alexa Fluor 647
Secondary antibody dilution:1:333

WB Suggested Anti-ACSL3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysateThere is BioGPS gene expression data showing that ACSL3 is expressed in HEK293T

Host: Rabbit
Target: ACSL3
Positive control (+): MCF7 (N10)
Negative control (-): Human lung (LU)
Antibody concentration: 1ug/ml

ACSL3 antibody - N-terminal region (ARP46453_P050) in Human 293T using Western Blot