SPTLC1 Antibody - middle region

Product Overview

• Article Name: SPTLC1 Antibody - middle region
• Biozol Catalog Number: ASB-ARP46722_P050
• Supplier Catalog Number: ARP46722_P050
• Alternative Catalog Number: ASB-ARP46722_P050-25UL,ASB-ARP46722_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPTLC1
• Alternative Names: HSN1, LBC1, LCB1, SPT1, SPTI, HSAN1

Product Description

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5-phosphate as a cofactor. SPTLC1 is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in SPTLC1 gene were identified in patients with hereditary sensory neuropathy type 1. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 53 kDa
• NCBI: 10558
• UniProt: O15269
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment. Isoforms containing the peptide sequence are present at 39 kDa and 35 kDa.

WB Suggested Anti-SPTLC1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human brain

Host: Rabbit
Target: SPTLC1
Positive control (+): MCF7 (N10)
Negative control (-): HepG2 (HG)
Antibody concentration: 1ug/ml

SPTLC1 antibody - middle region (ARP46722_P050) in Human Brain using Western Blot