DKK1 Antibody - C-terminal region

Product Overview

• Article Name: DKK1 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP48015_T100
• Supplier Catalog Number: ARP48015_T100
• Alternative Catalog Number: ASB-ARP48015_T100-25UL,ASB-ARP48015_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DKK1
• Alternative Names: SK, DKK-1

Product Description

DKK1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 29 kDa
• NCBI: 22943
• UniProt: O94907
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 2.5 ug/mL of the antibody was used in this experiment.

WB Suggested Anti-DKK1 Antibody Titration: 2.5ug/ml
Positive Control: HepG2 cell lysate

Lanes:
Lane 1: 30ug human PLC/PRF5 cell lysate
Lane 2: 30ug DKK1 PLC/PRF5 cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP Anti-rabbit-HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
DKK1 a
Submitted by:
Dr Frankie Ko Chi Fat, Lo-Kong Chan, Irene O.L. Ng, Judy Wai Ping Yam, Department of Pathology, The University of Hong Kong

DKK1 antibody - C-terminal region (ARP48015_T100) in Human HepG2 using Western Blot