MAPT Antibody - middle region

Product Overview

• Article Name: MAPT Antibody - middle region
• Biozol Catalog Number: ASB-ARP48103_P050
• Supplier Catalog Number: ARP48103_P050
• Alternative Catalog Number: ASB-ARP48103_P050-25UL,ASB-ARP48103_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MAPT
• Alternative Names: TAU, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, tau-40, FTDP-17, PPP1R103

Product Description

MAPT is differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. The mutations in the gene have been associated with several neurodegenerative disorders such as Alzheimers disease, Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimers disease, Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 79 kDa
• NCBI: 4137
• UniProt: P10636-8
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Sample Type:
A: Transgenic hTau P301L mouse cortex B: Tau-Ko mouse cortex C: WT mouse cortex Primary Antibody Dilution:
1:1000Secondary Antibody:
Anti-rabbit-biotin / Avidin-HRP Secondary Antibody Dilution:
1:1000 /1:2000 Color/Signal Descriptions:
Dark grey: MAPT Gene Name:
MAPTSubmitted by:
Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig

WB Suggested Anti-MAPT Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The peptide sequence is present in a 45 kDa isoform. The protein may be modified by acetylation, glycosylation, and phosphorylation.

Researcher: Max Holzer, Paul Flechsig Institut of Brain Research, University of Leipzig
Application: Western blotting
Species + Tissue/Cell type:
Lane 1: 10ug E.Coli: expressed human Tau-C
Lane 2: 10ug E.Coli: expressed human Tau-E
Lane 3: 10ug E.Coli: expressed human Tau-F
Lane 4: 10ug E.Coli: expressed human Tau-B
Lane 5: 10ug E.Coli: expressed human Fetal-Tau
Lane 6: 10ug E.Coli: expressed human Tau-D
Primary antibody dilution: 1:1000
Secondary antibody: Donkey anti-rabbit-HRP
Secondary antibody dilution: 1:10,000

MAPT antibody - middle region (ARP48103_P050) in Human HepG2 using Western Blot