EIF3E Antibody - middle region

Product Overview

• Article Name: EIF3E Antibody - middle region
• Biozol Catalog Number: ASB-ARP48176_P050
• Supplier Catalog Number: ARP48176_P050
• Alternative Catalog Number: ASB-ARP48176_P050-25UL,ASB-ARP48176_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF3E
• Alternative Names: INT6, EIF3S6, EIF3-P48, eIF3-p46

Product Description

EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD), It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 52kDa
• NCBI: 3646
• UniProt: P60228
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-EIF3E Antibody
Catalog Number: ARP48176_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic, nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-EIF3E Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate

Sample Type: 1. B8 mouse fibroblast cells, extraction only2. Human total cell extractPrimary Dilution: 1:1000Secondary Anitbody: HRP conjugated anti-rabbitImage Submitted By: Dr. Elah PickUniversity of Haifa at Oranim

Host: Rabbit
Target Name: EIF3E
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: EIF3E
Sample Tissue: Human Hela Whole Cell
Antibody Dilution: 1ug/ml

EIF3E antibody - middle region (ARP48176_P050) in Human HepG2 using Western Blot