VIM Antibody - N-terminal region

Product Overview

• Article Name: VIM Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP48225_P050
• Supplier Catalog Number: ARP48225_P050
• Alternative Catalog Number: ASB-ARP48225_P050-25UL,ASB-ARP48225_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human VIM
• Alternative Names: FLJ36605

Product Description

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 54kDa
• NCBI: 7431
• UniProt: P08670
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

submitted by:Carl LundinStanford UniversityThe Vimentin antibody ARP48225_P050 works well for visualization of the vimentin cytoskeleton (see attached images). We used PFA-fixed COS-7 cells and an antibody dilution of 1:400.

Sample Type:
Human brain stem cellsPrimary Antibody Dilution:
1:500Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution:
1:1000Color/Signal Descriptions:
VIM: Red DAPI:BlueGene Name:
VIMSubmitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Rabbit Anti-VIM Antibody
Catalog Number: ARP48225_P050
Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-VIM Antibody Titration: 0.2-1 ug/ml
Positive Control: Human brain

Host: Mouse
Target Name: VIM
Sample Tissue: Mouse Small Intestine
Antibody Dilution: 1ug/ml

VIM antibody - N-terminal region (ARP48225_P050) in Human brain using Western Blot