VISA Antibody - N-terminal region

Product Overview

• Article Name: VISA Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP49558_P050
• Supplier Catalog Number: ARP49558_P050
• Alternative Catalog Number: ASB-ARP49558_P050-25UL,ASB-ARP49558_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human VISA
• Alternative Names: IPS1, VISA, IPS-1, CARDIF

Product Description

Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 or by the cytoplasmic RNA helicases MDA5 and RIGI (ROBO3). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK) and IKKB (IKBKB), which activate NFKB, or TBK1 and IKKE (IKBKE), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1). For RIGI, the intermediary protein is mitochondria-bound VISA. Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3, MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK, MIM 600664) and IKKB (IKBKB, MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE, MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1, MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1, MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 56kDa
• NCBI: 57506
• UniProt: Q2HWT5
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Researcher: Dr. Safia Deddouche, London Research Institute
Application: IHC
Species+tissue/cell type:293T cells transfected with 3FLAG-VISA
Primary antibody dilution: 1:500
Secondary antibody: Anti-rabbit-Alexa 488
Secondary antibody dilution:1:500

WB Suggested Anti-VISA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Transfected 293T

Sample Type:
1: 20ug HEK293T no transfection, 2: 20ug HEK293T 3Flag-MAVS/VISAPrimary Antibody Dilution:
1:1000Secondary Antibody:
Anti-rabbit HRPSecondary Antibody Dilution:
1:1000Color/Signal Descriptions:
ARP49558-QC17479-WB-image-02Gene Name:
VISASubmitted by:
Dr. Safia Deddouche Immunobiology Laboratory Cancer Research UK London Research Institute Lincolns Inn Fields Laboratories 44 Lincolns Inn Fields London WC2A 3LY UKMAVS is supported by BioGPS gene expression data to be expressed in HEK293T

VISA antibody - N-terminal region (ARP49558_P050) in Transfected 293T using Western Blot