PIEZO2 Antibody - N-terminal region

Product Overview

• Article Name: PIEZO2 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP49682_P050
• Supplier Catalog Number: ARP49682_P050
• Alternative Catalog Number: ASB-ARP49682_P050-25UL,ASB-ARP49682_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human PIEZO2
• Alternative Names: DA3, DA5, MWKS, DAIPT, FAM38B, HsT748, HsT771, FAM38B2, C18orf30, C18orf58

Product Description

Piezos are large transmembrane proteins conserved among various species, all having between 24 and 36 predicted transmembrane domains. Piezo comes from the Greek piesi, meaning pressure. The PIEZO2 protein has a role in rapidly adapting mechanically activated (MA) currents in somatosensory neurons.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 77 kDa
• NCBI: 63895
• UniProt: Q9H5I5-3
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Host: Rabbit
Target Name: PIEZO2
Sample Type: 293T Whole Cell lysates
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: PIEZO2
Positive control (+): Human Ovary (OV)
Negative control (-): Human pancreas (PA)
Antibody concentration: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

PIEZO2 Antibody - N-terminal region (ARP49682_P050) in Human 293T using Western Blot