PORCN Antibody - N-terminal region

Product Overview

• Article Name: PORCN Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP49762_P050
• Supplier Catalog Number: ARP49762_P050
• Alternative Catalog Number: ASB-ARP49762_P050-25UL,ASB-ARP49762_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PORCN
• Alternative Names: PPN, DHOF, FODH, MG61, PORC

Product Description

PORCN belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the Porc family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the Porc family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 52 kDa
• NCBI: 64840
• UniProt: Q9H237-4
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Immunohistochemistry
Sample type:Mouse Uterus
- primary antibody dilution 1 in 300.
- secondary antibody dilution 1 in 1000.

WB Suggested Anti-PORCN Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate

Host: Rabbit
Target Name: PORCN
Sample Type: Hela Whole cell
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane
Gel Concentration: 0.12%

Host: Rabbit
Target Name: PORCN
Sample Tissue: Human Hela
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: PORCN
Positive control (+): Human lung (LU)
Negative control (-): Human liver (LI)
Antibody concentration: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Host: Rabbit
Target Name: PORCN
Sample Tissue: Human Lung Tumor
Antibody Dilution: 1ug/ml

PORCN antibody - N-terminal region (ARP49762_P050) in Human Hela using Western Blot