CYTB Antibody - N-terminal region

Product Overview

• Article Name: CYTB Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP50256_P050
• Supplier Catalog Number: ARP50256_P050
• Alternative Catalog Number: ASB-ARP50256_P050-25UL,ASB-ARP50256_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CYTB
• Alternative Names: MTCYB

Product Description

CYTB belongs to the cytochrome b family. It is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 42kDa
• NCBI: 4519
• UniProt: P00156
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-CYTB Antibody Titration: 0.2-1 ug/ml
Positive Control: Hela cell lysate

Host: Rabbit
Target Name: CYTB
Sample Type: MCF7
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12

Host: Rabbit
Target Name: CYTB
Sample Tissue: Human Fetal Liver
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: CYTB
Sample Tissue: Human MCF7 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: CYTB
Sample Tissue: Human OVCAR-3 Whole Cell
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

CYTB antibody - N-terminal region (ARP50256_P050) in Human Fetal Liver using Western Blot