UBA3 Antibody - N-terminal region

Product Overview

• Article Name: UBA3 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP50493_P050
• Supplier Catalog Number: ARP50493_P050
• Alternative Catalog Number: ASB-ARP50493_P050-25UL,ASB-ARP50493_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBA3
• Alternative Names: NAE2, UBE1C, hUBA3

Product Description

UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 52kDa
• NCBI: 9039
• UniProt: Q8TBC4
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Sample Type:
Human brain stem cellsPrimary Antibody Dilution:
1:500Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution:
1:1000Color/Signal Descriptions:
UBA3: Red DAPI:BlueGene Name:
UBA3Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

WB Suggested Anti-UBA3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Pancreas

Sample Type: 1. Human NT-2 cells (60ug)2. mouse brain extracts (80ug)
Primary antibody dilution: 2ug/ml
Secondary antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary antibody dilution: 1: 20,000
Image Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science _x005F _x005F

UBA3 antibody - N-terminal region (ARP50493_P050) in Human Pancreas using Western Blot