MED25 Antibody - N-terminal region

Product Overview

• Article Name: MED25 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP50698_P050
• Supplier Catalog Number: ARP50698_P050
• Alternative Catalog Number: ASB-ARP50698_P050-25UL,ASB-ARP50698_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: ChIP, WB
• Species Reactivity: Bovine, Canine, Guinea pig, Human, Mouse, Porcine, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED25
• Alternative Names: P78, ACID1, ARC92, BVSYS, PTOV2, CMT2B2, TCBAP0758

Product Description

This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 78 kDa
• NCBI: 81857
• UniProt: Q71SY5
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The peptide is present in isoforms of 87, 84, 78, 76 and 59 kDa but is absent from the canonical isoform of 90 kDa.

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

WB Suggested Anti-MED25 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human heart

MED25 antibody - N-terminal region (ARP50698_P050) in Human Heart using Western Blot