DBT Antibody - N-terminal region

Product Overview

• Article Name: DBT Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP51995_P050
• Supplier Catalog Number: ARP51995_P050
• Alternative Catalog Number: ASB-ARP51995_P050-25UL,ASB-ARP51995_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DBT
• Alternative Names: E2, E2B, BCATE2, BCKADE2, BCKAD-E2, BCKDH-E2, BCOADC-E2

Product Description

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 46kDa
• NCBI: 1629
• UniProt: P11182
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Immunohistochemistry of formalin-fixed, paraffin-embedded human spleen tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry of formalin-fixed, paraffin-embedded human skin tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

WB Suggested Anti-DBT Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: HT1080 cell lysateThere is BioGPS gene expression data showing that DBT is expressed in HT1080

Host: Rabbit
Target: DBT
Positive control (+): Human Liver (LI)
Negative control (-): HeLa Cell Lysate (HL)
Antibody concentration: 1ug/ml

Host: Rabbit
Target Name: DBT
Sample Tissue: Human THP-1 Whole Cell
Antibody Dilution: 0.5ug/ml

DBT antibody - N-terminal region (ARP51995_P050) in Human HT1080 using Western Blot