DNM1L Antibody - N-terminal region

Product Overview

• Article Name: DNM1L Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP52329_P050
• Supplier Catalog Number: ARP52329_P050
• Alternative Catalog Number: ASB-ARP52329_P050-25UL,ASB-ARP52329_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DNM1L
• Alternative Names: DLP1, DRP1, DVLP, EMPF, OPA5, EMPF1, DYMPLE, HDYNIV

Product Description

The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 82 kDa
• NCBI: 10059
• UniProt: O00429
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Application: IHC
Species+tissue/cell type: Mouse brain stem cells
Primary antibody dilution: 1:500
Secondary antibody: Goat anti-rabbit Alexa-Fluor 594
Secondary antibody dilution: 1:1000

WB Suggested Anti-DNM1L Antibody Titration: 0.2-1 ug/ml
Positive Control: Hela cell lysateDNM1L is supported by BioGPS gene expression data to be expressed in HeLa

Sample Type:A2. mouse brain extracts (80ug)
Primary Antibody Dilution:A2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:A1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical ScienceA

Host: Rabbit
Target: DNM1L
Positive control (+): A549 (N03)
Negative control (-): HepG2 (HG)
Antibody concentration: 3ug/ml

Host: Rabbit
Target Name: DNM1L
Sample Tissue: Human MCF7 Whole Cell
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Isoforms containing the peptide sequence are present at ~79-80 kDa.

Host: Rabbit
Target Name: DNM1L
Sample Tissue: Human DLD1 Whole Cell
Antibody Dilution: 1ug/ml

DNM1L antibody - N-terminal region (ARP52329_P050) in Human HeLa using Western Blot