UBE2F Antibody - N-terminal region

Product Overview

• Article Name: UBE2F Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP52515_P050
• Supplier Catalog Number: ARP52515_P050
• Alternative Catalog Number: ASB-ARP52515_P050-25UL,ASB-ARP52515_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2F
• Alternative Names: NCE2

Product Description

UBE2F belongs to the ubiquitin-conjugating enzyme family. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 21kDa
• NCBI: 140739
• UniProt: Q969M7
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Application: IHC
Species+tissue/cell type: Human brain stem cells
Primary antibody dilution: 1:500
Secondary antibody: Goat anti-rabbit Alexa-Fluor 594
Secondary antibody dilution: 1:1000

WB Suggested Anti-UBE2F Antibody Titration: 0.2-1 ug/ml
Positive Control: Hela cell lysate

Sample Type: 1. Human NT-2 cells (60ug)
Primary Antibody Dilution:A2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:A1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical ScienceA

UBE2F antibody - N-terminal region (ARP52515_P050) in Human HeLa using Western Blot