CHMP4B Antibody - middle region

Product Overview

• Article Name: CHMP4B Antibody - middle region
• Biozol Catalog Number: ASB-ARP53340_P050
• Supplier Catalog Number: ARP53340_P050
• Alternative Catalog Number: ASB-ARP53340_P050-25UL,ASB-ARP53340_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CHMP4B
• Alternative Names: SNF7, CTPP3, Shax1, CHMP4A, SNF7-2, VPS32B, CTRCT31, Vps32-2, C20orf178, dJ553F4.4

Product Description

This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 25kDa
• NCBI: 128866
• UniProt: Q9H444
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-CHMP4B antibody
Catalog Number: ARP53340
Formalin Fixed Paraffin Embedded Tissue: Human Kidney
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec

WB Suggested Anti-CHMP4B Antibody Titration: 0.2-1 ug/ml
Positive Control: THP-1 cell lysate

Host: Rabbit
Target Name: CHMP4B
Sample Tissue: Human Hela
Antibody Dilution: 1.0ug/ml

Host: Mouse
Target Name: CHMP4B
Sample Tissue: Mouse Skeletal Muscle
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: CHMP4B
Positive control (+): MCF7 (N10)
Negative control (-): Human liver (LI)
Antibody concentration: 1ug/ml

Host: Rabbit
Target Name: CHMP4B
Sample Tissue: Human Lung Tumor
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: CHMP4B
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 3ug/ml

Host: Rabbit
Target Name: CHMP4B
Sample Tissue: Human HCT116 Whole Cell
Antibody Dilution: 0.5ug/ml

CHMP4B antibody - middle region (ARP53340_P050) in Human Hela using Western Blot