IL13RA2 Antibody - N-terminal region

Product Overview

• Article Name: IL13RA2 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP53557_P050
• Supplier Catalog Number: ARP53557_P050
• Alternative Catalog Number: ASB-ARP53557_P050-25UL,ASB-ARP53557_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human IL13RA2
• Alternative Names: CT19, IL-13R, IL13BP, CD213A2

Product Description

IL13RA2 is closely related to Il13RA1, a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 44 kDa
• NCBI: 3598
• UniProt: Q14627
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

WB Suggested Anti-IL13RA2 Antibody Titration: 0.2-1 ug/ml
Positive Control: MCF7 cell lysate

Sample Type:
Lane 1: 60ug mouse CT26 lysate Lane 2: 60ug mouse MC38 lysate Lane 3: 60ug human HCT116 lysate Lane 4: 60ug mouse CT26 lysate + blocking peptide Lane 5: 60ug mouse MC38 lysate + blocking peptide Lane 6: 60ug human HCT116 lysate + blocking peptide Primary Antibody Dilution:
1:1000 Secondary Antibody:
Anti-rabbit-HRP Secondary Antibody Dilution:
1:5000 Color/Signal Descriptions:
IL13RA2 Gene Name:
Miranda A. Hallett, Vanderbilt University Medical Center Submitted by:

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

IL13RA2 antibody - N-terminal region (ARP53557_P050) in Human MCF-7 using Western Blot