APOE Antibody - N-terminal region

Product Overview

• Article Name: APOE Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP54284_P050
• Supplier Catalog Number: ARP54284_P050
• Alternative Catalog Number: ASB-ARP54284_P050-25UL,ASB-ARP54284_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Human, Mouse, Rat, Zebrafish
• Alternative Names: AD2, LPG, APO-E, ApoE4, LDLCQ5

Product Description

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 36 kDa
• NCBI: 348
• UniProt: P02649
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Application: IHC
Species+tissue/cell type: Mouse brain stem cells
Primary antibody dilution: 1:500
Secondary antibody: Goat anti-rabbit Alexa-Fluor 594
Secondary antibody dilution: 1:1000

APOE antibody - N-terminal region (ARP54284_P050) validated by WB using 721_B cell lysate at 1.0ug/ml.There is BioGPS gene expression data showing that APOE is expressed in 721_B

Sample Type:A2. mouse brain extracts (80ug)
3. rat brain extractA(80ug)
Primary Antibody Dilution:A2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:A1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical ScienceA

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

APOE antibody - N-terminal region (ARP54284_P050) in Human 721_B using Western Blot