IL1B Antibody - N-terminal region

Product Overview

• Article Name: IL1B Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP54323_P050
• Supplier Catalog Number: ARP54323_P050
• Alternative Catalog Number: ASB-ARP54323_P050-25UL,ASB-ARP54323_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Canine, Equine, Human, Mouse, Rabbit
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B
• Alternative Names: IL-1, IL1F2, IL1beta, IL1-BETA

Product Description

IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 17
• NCBI: 3553
• UniProt: P01584
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Human Macrophage

WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Hela cell lysate

WB Suggested Anti-IL1B Antibody
Positive Control: Lane 1: 80ug rat brain extract Lane 2: 80ug mouse brain extract
Primary Antibody Dilution : 1:500
Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience
Secondry Antibody Dilution : 1:20,000
Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science

Host: Rabbit
Target: IL1B
Positive control (+): Human lung (LU)
Negative control (-): Human Ovary (OV)
Antibody concentration: 3ug/ml

IL1B antibody - N-terminal region (ARP54323_P050) in Human HeLa using Western Blot