ALAS1 Antibody - N-terminal region

Product Overview

• Article Name: ALAS1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP54346_P050
• Supplier Catalog Number: ARP54346_P050
• Alternative Catalog Number: ASB-ARP54346_P050-25UL,ASB-ARP54346_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ALAS1
• Alternative Names: ALAS, MIG4, ALAS3, ALASH, ALAS-H

Product Description

Delta-aminolevulinate synthase (ALAS, EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 71 kDa
• NCBI: 211
• UniProt: P13196
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-ALAS1 antibody
Catalog Number: ARP54346
Formalin Fixed Paraffin Embedded Tissue: Human Liver
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec

WB Suggested Anti-ALAS1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Lung

Host: Rabbit
Target Name: ALAS1
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: ALAS1
Positive control (+): Human liver (LI)
Negative control (-): HepG2 (HG)
Antibody concentration: 0.5ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

Host: Rabbit
Target Name: ALAS1
Sample Tissue: Human 293T Whole Cell
Antibody Dilution: 1ug/ml

ALAS1 antibody - N-terminal region (ARP54346_P050) in Human Lung using Western Blot