GCLC Antibody - N-terminal region
Catalog Number:
ASB-ARP54576_P050
- Images (9)
| Article Name: | GCLC Antibody - N-terminal region |
| Biozol Catalog Number: | ASB-ARP54576_P050 |
| Supplier Catalog Number: | ARP54576_P050 |
| Alternative Catalog Number: | ASB-ARP54576_P050-25UL,ASB-ARP54576_P050-100UL |
| Manufacturer: | Aviva |
| Host: | Rabbit |
| Category: | Proteine/Peptide |
| Application: | IHC, WB |
| Species Reactivity: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human GCLC |
| Alternative Names: | GCL, GCS, GLCL, GLCLC |
| Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment. Peptide is also present in an isoform of ~28 kDa. |
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GCLC antibody - N-terminal region (ARP54576_P050) Catalog Number: ARP54576_P050 Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml.A: - blocking peptide.B: + blocking peptide. |
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WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml Positive Control: Human Liver |
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Lanes: 1: 40ug mouse heart lysate, 2: 40ug mouse heart lysate, 3: 40ug mouse heart lysate, 4: 40ug mouse heart lysate, 5: 40ug mouse heart lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:10000 Gene Name: GCLC Submitted by: Anonymous |
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Host: Rabbit Target Name: GCLC Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml |
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Host: Mouse Target Name: GCLC Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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GCLC antibody - N-terminal region (ARP54576_P050) in Human Liver using Western Blot |
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