GCLC Antibody - middle region

Product Overview

• Article Name: GCLC Antibody - middle region
• Biozol Catalog Number: ASB-ARP54577_P050
• Supplier Catalog Number: ARP54577_P050
• Alternative Catalog Number: ASB-ARP54577_P050-25UL,ASB-ARP54577_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLC
• Alternative Names: GCL, GCS, GLCL, GLCLC

Product Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 73 kDa
• NCBI: 2729
• UniProt: P48506
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Spleen

WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Placenta

Host: Rabbit
Target Name: GCLC
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml

Host: Mouse
Target Name: GCLC
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

Host: Rabbit
Target Name: GCLC
Sample Tissue: Human THP-1 Whole Cell
Antibody Dilution: 3ug/ml

GCLC antibody - middle region (ARP54577_P050) in Human Placenta using Western Blot