GRN Antibody - middle region

Product Overview

• Article Name: GRN Antibody - middle region
• Biozol Catalog Number: ASB-ARP54641_P050
• Supplier Catalog Number: ARP54641_P050
• Alternative Catalog Number: ASB-ARP54641_P050-25UL,ASB-ARP54641_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Human, Rabbit
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human GRN
• Alternative Names: GEP, GP88, PEPI, PGRN, CLN11, PCDGF

Product Description

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 47 kDa
• NCBI: 2896
• UniProt: P28799-2
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-GRN Antibody
Catalog Number: ARP54641_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Host: Rabbit
Target Name: GRN
Sample Type: Thyroid Tumor lysates
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: GRN
Positive control (+): Human Lymph Node (LN)
Negative control (-): Human Pancreas (PA)
Antibody concentration: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 62 kDa isoform is identified, and a second isoform of 47 kDa, and a third isoform of 44 kDa are also present in some samples.

GRN Antibody - middle region (ARP54641_P050) in Human Thyroid Tumor using Western Blot