ICA1 Antibody - N-terminal region

Product Overview

• Article Name: ICA1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP54742_P050
• Supplier Catalog Number: ARP54742_P050
• Alternative Catalog Number: ASB-ARP54742_P050-25UL,ASB-ARP54742_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, IP, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ICA1
• Alternative Names: ICA69, ICAp69

Product Description

ICA1 is a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogrens syndrome. This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogrens syndrome. Alternatively spliced variants which encode different protein isoforms have been described, however, not all variants have been fully characterized.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 55kDa
• NCBI: 3382
• UniProt: Q05084
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

ICA1 antibody - N-terminal region (ARP54742_P050)
Catalog Number: ARP54742_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

ICA1 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with ARP54742_P050 with 1:200 dilution. Western blot was performed using ARP54742_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: ICA1 IP with ARP54742_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.

WB Suggested Anti-ICA1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Hela cell lysate

Host: Rabbit
Target Name: ICA1
Sample Type: Human HepG2
Antibody Dilution: 1.0ug/mlThere is BioGPS gene expression data showing that ICA1 is expressed in HepG2

ICA1 antibody - N-terminal region (ARP54742_P050) in Human HeLa using Western Blot