GRK5 Antibody - middle region

Product Overview

• Article Name: GRK5 Antibody - middle region
• Biozol Catalog Number: ASB-ARP54750_P050
• Supplier Catalog Number: ARP54750_P050
• Alternative Catalog Number: ASB-ARP54750_P050-25UL,ASB-ARP54750_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GRK5
• Alternative Names: GPRK5, FP2025

Product Description

GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs).This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 68kDa
• NCBI: 2869
• UniProt: P34947
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

GRK5 antibody - middle region (ARP54750_P050)
Catalog Number: ARP54750_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasm and membrane of alveolar macrophages
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-GRK5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human brain

Lanes:
Lanes 1-3: 40 ug tobacco hornworm larvae intestine lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
GRK5
Submitted by:
Dra. Helena Porta Ducoing and Biol. Gladys Jimenez Nopala

Lanes:
Lane1: 1ug human GRK5 protein
Lane2: 0.25ug human GRK5 protein
Lane3: 0.1ug human GRK5 protein
Lane4: 5ug rat striatum homogenate
Lane5: 5ug rat striatum cytosol
Lane6: 5ug rat striatum light membrane fraction
Lane7: 5ug rat striatum synaptic membrane fraction
Lane8: 5ug rat striatum crude synaptic vesicle fraction
Lane9: 5ug rat striatum homogenate
Lane10: 5ug rat striatum cytosol
Lane11: 5ug rat striatum light membrane fraction
Lane12: 5ug rat striatum synaptic membrane fraction
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:15000
Gene Name:
GRK5
Submitted by:
Eugenia V Gurevich, Vanderbilt University

Sample Type:
Lane 1: 20ug mouse left ventricle heart lysate Primary Antibody Dilution:
1:1000 Secondary Antibody:
Anti-rabbit-HRP Secondary Antibody Dilution:
1:5000 Color/Signal Descriptions:
GRK5 Gene Name:
Kathleen Gabrielson Submitted by:

Host: Mouse
Target Name: GRK5
Sample Tissue: Mouse Spleen
Antibody Dilution: 1ug/ml

GRK5 antibody - middle region (ARP54750_P050) in Human Brain using Western Blot