LDHA Antibody - N-terminal region

Product Overview

• Article Name: LDHA Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP54776_P050
• Supplier Catalog Number: ARP54776_P050
• Alternative Catalog Number: ASB-ARP54776_P050-25UL,ASB-ARP54776_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LDHA
• Alternative Names: LDHM, GSD11, PIG19, HEL-S-133P

Product Description

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 37 kDa
• NCBI: 3939
• UniProt: P00338
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Anti-LDHA antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. LDHA Antibody concentration 2.5 ug/ml.

WB Suggested Anti-LDHA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Placenta

LDHA antibody - N-terminal region (ARP54776_P050) validated by WB using LdhC knockout mouse sperm, LdhA transgenic kidney, IdhA transgenic testis, LdhC knockout testis at 1:1000.

Host: Rabbit
Target Name: LDHA
Sample Tissue: Human Hela
Antibody Dilution: 1.0ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Isoforms of ~35 kDa and 30 kDa also contain the peptide.

Host: Rabbit
Target Name: LDHA
Sample Tissue: Human Ovary Tumor
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: LDHA
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

LDHA antibody - N-terminal region (ARP54776_P050) in Human Hela using Western Blot