CCT5 Antibody - N-terminal region

Product Overview

• Article Name: CCT5 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP54825_P050
• Supplier Catalog Number: ARP54825_P050
• Alternative Catalog Number: ASB-ARP54825_P050-25UL,ASB-ARP54825_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CCT5
• Alternative Names: CCTE, HEL-S-69, PNAS-102, CCT-epsilon, TCP-1-epsilon

Product Description

CCT5 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 60kDa
• NCBI: 22948
• UniProt: P48643
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-CCT5 Antibody
Catalog Number: ARP54825_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-CCT5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human brain

Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Application: Western blotting
Species+tissue/cell type: Mouse WT brain and Rat brainHow many ugs of tissue/cell lysate run on the gel: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)
Primary antibody dilution: 2ug/ml
Secondary antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary antibody dilution: 1: 20,000

Lanes:
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)
Primary Antibody Dilution:
2ug/ml
Secondary Antibody:
IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:
1: 20,000
Gene Name:
CCT5
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
CCT5 is supported by BioGPS gene expression data to be expressed in HEK293

CCT5 antibody - N-terminal region (ARP54825_P050) in Human Brain using Western Blot