SENP3 Antibody - N-terminal region

Product Overview

• Article Name: SENP3 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP55322_P050
• Supplier Catalog Number: ARP55322_P050
• Alternative Catalog Number: ASB-ARP55322_P050-25UL,ASB-ARP55322_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IF, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SENP3
• Alternative Names: SSP3, Ulp1, SMT3IP1

Product Description

SENP3 is a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 65kDa
• NCBI: 26168
• UniProt: Q9H4L4
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

SENP3 antibody - N-terminal region (ARP55322_P050) in HeLa cells using Immunofluorescence.
Antibody concentration: 2 ug/ml
Data submitted by: Michael Matunis, Johns Hopkins University
SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa

WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 721_B cell lysateSENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

Sample Type: 1. Rat Liver (200ug)
2. Rat Brain (200ug)
3. Rat Testis (200ug)
4. Rat Kidney (200ug)
5.Rat Liver nuclei (-) no HA-SUMO2-WME
6.Rat Liver nuclei (+) incubated with 100 ng HA-SUMO2-WME
Primary Dilution: 1:1000
Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit
Secondary Dilution: 1:1000
Image Submitted by: Andreia Carvalho
IBMC-OBF, PortugalSee Customer Feedback tab for detailed information.

Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa

Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa

SENP3 antibody - N-terminal region (ARP55322_P050) in Human 721_B using Western Blot