BCKDHA Antibody - N-terminal region

Article Name:	BCKDHA Antibody - N-terminal region
Biozol Catalog Number:	ASB-ARP56119_P050
Supplier Catalog Number:	ARP56119_P050
Alternative Catalog Number:	ASB-ARP56119_P050-25UL,ASB-ARP56119_P050-100UL
Manufacturer:	Aviva
Host:	Rabbit
Category:	Proteine/Peptide
Application:	IHC, WB
Species Reactivity:	Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Immunogen:	The immunogen is a synthetic peptide directed towards the N terminal region of human BCKDHA
Alternative Names:	MSU, MSUD1, OVD1A, BCKDE1A

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD, EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2, MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3, MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-675 BI910860.1 12-686 676-1196 BG742673.1 80-600 1197-1731 BM702667.1 48-582 1732-1763 BE223026.1 1-32 c 1764-1781 BQ018849.1 1-18 c

Clonality:	Polyclonal
Concentration:	0.5 mg/ml
Molecular Weight:	50kDa
NCBI:	593
UniProt:	P12694
Form:	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

	Rabbit Anti-BCKDHA Antibody Catalog Number: ARP56119_P050 Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasmic in alveolar type I & II cells Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
	WB Suggested Anti-BCKDHA Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: MCF7 cell lysateBCKDHA is supported by BioGPS gene expression data to be expressed in MCF7
	Host: Rabbit Target Name: SERPINA3 Sample Type: Human Adult Placenta Antibody Dilution: 1.0ug/ml
	Host: Rabbit Target: BCKDHA Positive control (+): MCF7 (N10) Negative control (-): HepG2 (HG) Antibody concentration: 1ug/ml
	Host: Rabbit Target Name: BCKDHA Sample Tissue: Human Jurkat Whole Cell Antibody Dilution: 1ug/ml
	BCKDHA antibody - N-terminal region (ARP56119_P050) in Human MCF-7 using Western Blot