NDUFS1 Antibody - middle region

Product Overview

• Article Name: NDUFS1 Antibody - middle region
• Biozol Catalog Number: ASB-ARP56609_P050
• Supplier Catalog Number: ARP56609_P050
• Alternative Catalog Number: ASB-ARP56609_P050-25UL,ASB-ARP56609_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NDUFS1
• Alternative Names: CI-75k, MC1DN5, CI-75Kd, PRO1304

Product Description

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 79 kDa
• NCBI: 4719
• UniProt: P28331
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The protein may be modified by acetylation.

WB Suggested Anti-NDUFS1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Fetal Liver

Human nuclear lysates

Lanes:
Lane1: 30 ug Human liver
Lane2: 30 ug Rat liver
Lane3: 30 ug Mouse (wild-type) liver
Lane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liver
Lane5: 30 ug Human muscle
Lane6: 30 ug Rat muscle
Lane7: 30 ug Mouse muscle
Primary Antibody Dilution:
1:1000
Secondary Antibody:

Secondary Antibody Dilution:
1:10000
Gene Name:
NDUFS1
Submitted by:
Dr. Bruno Guigas, PhD, Dept. of Molecular Cell Biology, Leiden University Medical Center

NDUFS1 antibody - middle region (ARP56609_P050) in Human Fetal Liver using Western Blot