SIAH2 Antibody - N-terminal region

Product Overview

• Article Name: SIAH2 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP58071_P050
• Supplier Catalog Number: ARP58071_P050
• Alternative Catalog Number: ASB-ARP58071_P050-25UL,ASB-ARP58071_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SIAH2
• Alternative Names: hSiah2

Product Description

SIAH2 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia.This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 35 kDa
• NCBI: 6478
• UniProt: O43255
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Hela cell lysate

Lanes:
1: 50ug HEK293T lysate, 2: 50ug A549 lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
SIAH2
Submitted by:
Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena

Host: Rabbit
Target Name: SIAH2
Sample Type: HepG2
Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in HepG2

Host: Rabbit
Target Name: SIAH2
Sample Type: MCF7
Antibody Dilution: 1.0ug/mlSIAH2 is supported by BioGPS gene expression data to be expressed in MCF7

Host: Rabbit
Target Name: SIAH2
Sample Type: Hela
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

Host: Rabbit
Target Name: SIAH2
Sample Tissue: Human OVCAR-3 Whole Cell
Antibody Dilution: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.

SIAH2 antibody - N-terminal region (ARP58071_P050) in Human HeLa using Western Blot