MED14 Antibody - middle region

Product Overview

• Article Name: MED14 Antibody - middle region
• Biozol Catalog Number: ASB-ARP58139_P050
• Supplier Catalog Number: ARP58139_P050
• Alternative Catalog Number: ASB-ARP58139_P050-25UL,ASB-ARP58139_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: ChIP, WB
• Species Reactivity: Human, Mouse, Rabbit
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED14
• Alternative Names: CSRP, RGR1, CRSP2, EXLM1, CXorf4, CRSP150, DRIP150, TRAP170

Product Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 161 kDa
• NCBI: 9282
• UniProt: O60244
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

WB Suggested Anti-MED14 Antibody Titration: 0.2-1 ug/ml
Positive Control: A549 cell lysateMED14 is strongly supported by BioGPS gene expression data to be expressed in Human A549 cells

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. In addition to the 161 kDa canonical isoform, a 127 kDa putative isoform is present in some samples.

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