SOD2 Antibody - N-terminal region

Product Overview

• Article Name: SOD2 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP58529_P050
• Supplier Catalog Number: ARP58529_P050
• Alternative Catalog Number: ASB-ARP58529_P050-25UL,ASB-ARP58529_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SOD2
• Alternative Names: IPOB, IPO-B, MNSOD, MVCD6, GClnc1, Mn-SOD

Product Description

SOD2 is a member of the iron/manganese superoxide dismutase family. SOD2 is a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene encoding SOD2 have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 25 kDa
• NCBI: 6648
• UniProt: P04179
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-SOD2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Placenta

WB Suggested Anti-SOD2 antibody
Titration: 0.4 ug/ml
Positive Control: Rat dorsal medulla brain & cortax + hypothalamus extract

Lanes:
1. 40ug rat dorsal medulla brain extract 2. 20 ug rat cortax + hypothalamus mitochondria extract
Primary Antibody Dilution:
1:2500
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
SOD2
Submitted by:
Manisha Nautiyal, Hypertension and Vascular Research Center, Wake Forest University School of Medicine

Lanes:
1. 40 ug HK2 cell (kidney proximal tubular cell line) 2. 40 ug H2O2 treated human HK2 Cell lysate
3. 40 ug H2O2 treated human HK2 Cell lysate
4. 40 ug H2O2 treated human HK2 Cell lysate
5. 40 ug H2O2 treated human HK2 Cell lysate
6. 40 ug H2O2 treated human HK2 Cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit HRP
Secondary Antibody Dilution:
1:2000
Gene Name:
SOD2
Submitted by:
The University of Queensland, School of Medicine, Centre for Kidney Disease Research

Host: Rabbit
Target Name: NOP56
Sample Type: Human Fetal Muscle
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: NOP56
Sample Type: Human Fetal Brain
Antibody Dilution: 1.0ug/ml

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Host: Rabbit
Target Name: SOD2
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml

SOD2 antibody - N-terminal region (ARP58529_P050) in Human Placenta using Western Blot