BUB3 Antibody - N-terminal region

Product Overview

• Article Name: BUB3 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP58599_P050
• Supplier Catalog Number: ARP58599_P050
• Alternative Catalog Number: ASB-ARP58599_P050-25UL,ASB-ARP58599_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IF, IHC, WB
• Species Reactivity: Bovine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BUB3
• Alternative Names: BUB3L, hBUB3

Product Description

BUB3 is required for kinetochore localization of BUB1.This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 36kDa
• NCBI: 9184
• UniProt: A6NJ42
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Sample Type:
HCT116
Primary Antibody Dilution:
4 ug/ml
Secondary Antibody:
Anti-rabbit Alexa 546
Secondary Antibody Dilution:
2 ug/ml
Gene Name:
BUB3

Rabbit Anti-BUB3 Antibody
Catalog Number: ARP58599_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-BUB3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: PANC1 cell lysate.BUB3 is strongly supported by BioGPS gene expression data to be expressed in PANC1

Host: Rabbit
Target Name: BUB3
Sample Type: 293T
Antibody Dilution: 1.0ug/mlBUB3 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells

Host: Mouse
Target Name: BUB3
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml

BUB3 antibody - N-terminal region (ARP58599_P050) in Human PANC1 using Western Blot