Wnt3a Antibody - C-terminal region

Product Overview

• Article Name: Wnt3a Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP58819_P050
• Supplier Catalog Number: ARP58819_P050
• Alternative Catalog Number: ASB-ARP58819_P050-25UL,ASB-ARP58819_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse
• Alternative Names: vt, Wnt-, Wnt-3a

Product Description

Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 38kDa
• NCBI: 22416
• UniProt: P27467
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Immunohistochemistry with Placenta tissue at an antibody concentration of Dilution: 1:500 using anti-Wnt3a antibody (ARP58819_P050)

WB Suggested Anti-Wnt3a Antibody Titration: 1 ug/ml
Positive Control: Mouse Heart lysate

Host: Rabbit
Target Name: Wnt3a
Sample Type: Mouse Heart
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane
Gel Concentration: 0.12%

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

Wnt3a antibody - C-terminal region (ARP58819_P050) in Mouse Heart using Western Blot