VCP Antibody - C-terminal region

Product Overview

• Article Name: VCP Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP59365_P050
• Supplier Catalog Number: ARP59365_P050
• Alternative Catalog Number: ASB-ARP59365_P050-25UL,ASB-ARP59365_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, IP, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human VCP
• Conjugation: Unconjugated
• Alternative Names: p97, TERA, CDC48, FTDALS6

Product Description

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 89kDa
• NCBI: 7415
• UniProt: P55072
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-VCP antibody
Catalog Number: ARP59365
Formalin Fixed Paraffin Embedded Tissue: Human Adult Skin
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 a€'' 2.0 sec

Rabbit Anti-VCP Antibody
Catalog Number: ARP59365_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic, nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Amount and Sample Type:
500 ug Human NT2 cell lysate
Amount of IP Antibody:
6 ug
Primary Antibody:
VCP
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:5000
Gene Name:
VCP
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

WB Suggested Anti-VCP Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Liver

Lanes:
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)
Primary Antibody Dilution:
2ug/ml
Secondary Antibody:
IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:
1: 20,000
Gene Name:
VCP
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

VCP antibody - C-terminal region (ARP59365_P050) in Human Liver using Western Blot