RPS27A Antibody - middle region

Product Overview

• Article Name: RPS27A Antibody - middle region
• Biozol Catalog Number: ASB-ARP59416_P050
• Supplier Catalog Number: ARP59416_P050
• Alternative Catalog Number: ASB-ARP59416_P050-25UL,ASB-ARP59416_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPS27A
• Alternative Names: UBC, S27A, CEP80, UBA80, HEL112, UBCEP1, UBCEP80

Product Description

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin, similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 18 kDa
• NCBI: 6233
• UniProt: P62979
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 0.3 ug/mL of the antibody was used in this experiment. Protein is ubiquitinated which adds 8 kDa to the molecular weight.

WB Suggested Anti-RPS27A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 293T cell lysateRPS27A is supported by BioGPS gene expression data to be expressed in HEK293T

Host: Mouse
Target Name: RPS27A
Sample Tissue: Mouse Kidney
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: RPS27A
Positive control (+): 293T (2T)
Negative control (-): Human Placenta (PL)
Antibody concentration: 1ug/ml

RPS27A antibody - middle region (ARP59416_P050) in Human 293T using Western Blot