NOS2 Antibody - N-terminal region

Product Overview

• Article Name: NOS2 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP63000_P050
• Supplier Catalog Number: ARP63000_P050
• Alternative Catalog Number: ASB-ARP63000_P050-25UL,ASB-ARP63000_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Human
• Alternative Names: NOS, INOS, NOS2A, HEP-NOS

Product Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 110kDa
• NCBI: 4843
• UniProt: P35228
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-NOS2 Antibody
Catalog Number: ARP63000_P050
Formalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-NOS2 Antibody
Titration: 1.0 ug/ml
Positive Control: Jurkat Whole Cell

Lanes:
Lane1: 30 ug human placental tissue lysate
Lane2: 30 ug human placental tissue lysate
Lane3: 30 ug human placental tissue lysate
Lane4: 30 ug human placental tissue lysate
Lane5: 20 ug human myometrial tissue lysate
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
Nitric oxide synthase 2, inducible (NOS2)
Submitted by:
Hiten Mistry, Ania Czajka and Marta Hentschke Ribeiro, Kings College London

Researcher: Hiten Mistry, Ania Czajka and Marta Hentschke Ribeiro, Kings College London
Application: Western blotting
Species+tissue/cell type: Human placental and myometrial tissue lysate
How many ugsof tissue/cell lysate run on the gel:1: 50 ug human placental tissue lysate2: 40 ug human placental tissue lysate3: 30 ug human placental tissue lysate4: 20 ug human placental tissue lysate5: 10 ug human placental tissue lysate6: 20 ug human myometrial tissue lysate
Primary antibody dilution: 1:500
Secondary antibody: Goat anti-rabbit HRP
Secondary antibody dilution: 1:10000

Sample Type:
Human placental tissue Primary Antibody Dilution:
1:50Secondary Antibody:
Goat anti rabbit-HRP Secondary Antibody Dilution:
1:10,000Color/Signal Descriptions:
Brown: NOS2Purple: HaemotoxylinGene Name:
NOS2Submitted by:
Dr. Hiten D. Mistry and Anna Czajka, Kings College London, Lesia Kurlak, University of Nottingham

NOS2 antibody - N-terminal region (ARP63000_P050) in Human Jurkat using Western Blot