TNFAIP6 Antibody - C-terminal region

Product Overview

• Article Name: TNFAIP6 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP63662_P050
• Supplier Catalog Number: ARP63662_P050
• Alternative Catalog Number: ASB-ARP63662_P050-25UL,ASB-ARP63662_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Alternative Names: TSG6, TSG-6

Product Description

The protein encoded by this gene is a secretory protein that contains a hyaluronan-binding domain, and thus is a member of the hyaluronan-binding protein family. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable complex with inter-alpha-inhibitor (I alpha I), and thus enhance the serine protease inhibitory activity of I alpha I, which is important in the protease network associated with inflammation. This gene can be induced by proinflammatory cytokines such as tumor necrosis factor alpha and interleukin-1. Enhanced levels of this protein are found in the synovial fluid of patients with osteoarthritis and rheumatoid arthritis.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 31 kDa
• NCBI: 7130
• UniProt: P98066
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

WB Suggested Anti-TNFAIP6 Antibody
Titration: 1.0 ug/ml
Positive Control: HCT15 Whole Cell

Host: Rabbit
Target: TNFAIP6
Positive control (+): Human stomach (ST)
Negative control (-): Human Ovary (OV)
Antibody concentration: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

TNFAIP6 antibody - C-terminal region (ARP63662_P050) in Human HCT15 using Western Blot