IL4I1 Antibody - C-terminal region

Product Overview

• Article Name: IL4I1 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP65454_P050
• Supplier Catalog Number: ARP65454_P050
• Alternative Catalog Number: ASB-ARP65454_P050-25UL,ASB-ARP65454_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human IL4I1
• Alternative Names: LAO, FIG1, LAAO, hIL4I1

Product Description

The protein encoded by this gene shares the limited similarity with L-amino acid oxidase, and contains the conserved key amino acid residues thought to be involved in catalysis and binding of the flavin adenine dinucleotide cofactor. The expression of this gene can be induced by interleukin 4 in B cells. Two alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 63 kDa
• NCBI: 259307
• UniProt: Q96RQ9
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

WB Suggested Anti-IL4I1 Antibody
Titration: 1.0 ug/ml
Positive Control: Hela Whole Cell

Host: Rabbit
Target: IL4I1
Positive control (+): RPMI-8226 (N12)
Negative control (-): A549 (N03)
Antibody concentration: 3ug/ml

Host: Rabbit
Target Name: IL4I1
Sample Tissue: Human HCT116 Whole Cell
Antibody Dilution: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

IL4I1 Antibody - C-terminal region (ARP65454_P050) in Human HeLa using Western Blot