CH25H Antibody - C-terminal region

Product Overview

• Article Name: CH25H Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP65536_P050
• Supplier Catalog Number: ARP65536_P050
• Alternative Catalog Number: ASB-ARP65536_P050-25UL,ASB-ARP65536_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human CH25H
• Alternative Names: C25H

Product Description

This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 32 kDa
• NCBI: 9023
• UniProt: O95992
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-CH25H Antibody
Titration: 1.0 ug/ml
Positive Control: Fetal Brain

Host: Rabbit
Target Name: CH25H
Sample Tissue: Human MCF7 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: CH25H
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

CH25H Antibody - C-terminal region (ARP65536_P050) in Human Fetal Brain using Western Blot