MDM2 Antibody - middle region

Product Overview

• Article Name: MDM2 Antibody - middle region
• Biozol Catalog Number: ASB-ARP74981_P050
• Supplier Catalog Number: ARP74981_P050
• Alternative Catalog Number: ASB-ARP74981_P050-25UL,ASB-ARP74981_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IP, WB
• Species Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human MDM2
• Alternative Names: HDMX, LSKB, hdm2, ACTFS

Product Description

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 55 kDa
• NCBI: 4193
• UniProt: Q00987-9
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Rabbit Anti-MDM2 Antibody
Catalog Number: ARP74981_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

MDM2 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with ARP74981_P050 with 1:200 dilution. Western blot was performed using ARP74981_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: MDM2 IP with ARP74981_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.

Host: Rabbit
Target Name: MDM2
Sample Type: Fetal Kidney lysates
Antibody Dilution: 1.0ug/ml

MDM2 Antibody (ARP74981_P050) in Human Fetal Kidney using Western Blot