STX4 Antibody - N-terminal region

Product Overview

• Article Name: STX4 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP85503_P050
• Supplier Catalog Number: ARP85503_P050
• Alternative Catalog Number: ASB-ARP85503_P050-25UL,ASB-ARP85503_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human STX4
• Alternative Names: STX4A, p35-2

Product Description

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 34 kDa
• NCBI: 6810
• UniProt: Q12846
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ig of the indicated Syntaxin-4 antibodies pre-coupled to either protein G or protein A magnetic beads. Samples were washed and processed for immunoblot with the Syntaxin-4 antibody. For immunoblot the antibody was used at a dilution of 1/200. The Ponceau stained transfer of the blot is shown. SM=10% starting material, UB=10% unbound fraction, IP=immunoprecipitate. Data provided courtesty of the Ycharos Antibody Characterization through Open Science group. Additional data can be found at https://ycharos.com/data/.

50 ug of HAP1 WT and STX4 KO lysates were subjected to SDS-PAGE followed by immunoblotting with a dilution of 1/1000. The second image shows Ponceau S staining of the transferred blot as a loading control experiment. Data provided courtesty of the Ycharos Antibody Characterization through Open Science group. Additional data can be found at https://ycharos.com/data/.

Host: Rabbit
Target Name: STX4
Sample Tissue: Human Jurkat Whole Cell lysates
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

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