NME1 Antibody - N-terminal region

Product Overview

• Article Name: NME1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-AVARP00009_P050
• Supplier Catalog Number: AVARP00009_P050
• Alternative Catalog Number: ASB-AVARP00009_P050-25UL,ASB-AVARP00009_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: DOT, IHC, WB
• Species Reactivity: Bovine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NME1
• Alternative Names: NB, AWD, NBS, GAAD, NDKA, NM23, NDPKA, NDPK-A, NM23-H1

Product Description

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of A (encoded by this gene) and B (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of A (encoded by this gene) and B (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 17kDa
• NCBI: 4830
• UniProt: P22392
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Human kidney

Anti-NME1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Primary Antibody concentration 5 ug/ml.

DB Suggested Anti-Nme1 antibody
Titration: 0.5 ug/ml
Positive Control: Recomninant human NME2 protein

Anti-NME1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Primary Antibody concentration 5 ug/ml.

NME1 antibody - N-terminal region (AVARP00009_P050) validated by WB using Fetal Heart Lysate at 1ug/ml.

Lanes:
1. 20 ug murine 4T1 primary breast tumor cell lysate
2. 20 ug human MDA-MB-231 cell lysate
3. 20 ug murine 4T1 primary breast tumor cell lysate
Primary Antibody Dilution:
1:4000
Secondary Antibody:
Goat anti-rabbit-Alexa Fluor 680
Secondary Antibody Dilution:
1:25,000
Gene Name:
NME1
Submitted by:
Anonymous

Lanes:
1. 20 ug murine 4T1 primary breast tumor cell lysate
2. 20 ug human MDA-MB-231 cell lysate
3. 20 ug murine 4T1 primary breast tumor cell lysate
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Goat anti-rabbit-Alexa Fluor 680
Secondary Antibody Dilution:
1:25,000
Gene Name:
NME1
Submitted by:
Anonymous

NME1 antibody - N-terminal region (AVARP00009_P050) in Human Fetal heart using Western Blot