AKT1 Antibody - N-terminal region

Product Overview

• Article Name: AKT1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-AVARP06008_P050
• Supplier Catalog Number: AVARP06008_P050
• Alternative Catalog Number: ASB-AVARP06008_P050-25UL,ASB-AVARP06008_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Goat, Human, Mouse, Porcine, Rat, Sheep
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AKT1
• Alternative Names: AKT, PKB, RAC, PRKBA, PKB-ALPHA, RAC-ALPHA

Product Description

AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 56kDa
• NCBI: 207
• UniProt: P31749
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-AKT1 Antibody
Catalog Number: AVARP06008_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasm, Plasma membrane
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-AKT1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Small Intestine

WB Suggested Anti-AKT1 Antibody
Positive Control: Lane 1: 50ug preterm baboon muscle homogenate Lane 2: 50ug preterm baboon muscle homogenate Lane 3: 50ug term baboon muscle homogenate Lane 4: 50ug term baboon muscle homogenate Lane 5: 50ug adult baboon muscle homogenate Lane 6: 50ug adult baboon muscle homogenate
Primary Antibody Dilution : 1:1666
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:1200
Submitted by: Cynthia Blanco, University of Texas Health Science Center

Lanes:
1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laevis embryos lysate (HB buffer)
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat Anti-Rabbit AP
Secondary Antibody Dilution:
1:20,000
Gene Name:
AKT1
Submitted by:
Dr. Rosa Carotenuto, Department of Structural and Functional Biology, University of Naples Federico II

AKT1 antibody - N-terminal region (AVARP06008_P050) in Human Small Intestine using Western Blot