Usually, the Transcriptional regulation element or 5-promoter region of the gene of interest is cloned upstream of Luciferase, or the 3- UTR region is cloned downstream of Luciferase to construct a Reporter gene plasmid, and then transfect the cell, treat the cell with appropriate drugs, and then Lytic cells. The Transcriptional regulation effect of drug treatment on the target gene is judged by detecting the activity of Luciferase.
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