CheKine(TM) Micro Hexokinase(HK) Assay Kit provides a simple and easy Colorimetric assay for measuring HK in samples.
Range:
31.25-2000µM
Tag:
HK
Application Notes:
CheKine(TM) Micro Hexokinase (HK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric HK detection method.HK catalyzes glucose synthesis of 6-phosphoglucose, and 6-phosphoglucose dehydrogenase further catalyzes 6-phosphoglucose dehydrogenation to NADH, which reduces WST-8 to orange formazan, with the maximum absorption peak detected at around 450 nm. The enzyme activity was calculated by detecting the increase rate of light absorption at 450 nm
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