CheKine(TM) Micro Total Iron-Binding Capacity (TIBC) Assay Kit
Biozol Catalog Number:
ASC-KTB2150
Supplier Catalog Number:
KTB2150
Alternative Catalog Number:
ASC-KTB2150-96
Manufacturer:
Abbkine Scientific
Category:
Kits/Assays
CheKine(TM) Micro Total Iron-Binding Capacity (TIBC ) Assay Kit provides a simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples.
Tag:
Total Iron Binding Capacity ,TIBC
Application Notes:
CheKine(TM) Micro Total Iron-Binding Capacity (TIBC ) Assay Kit provides a simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples. The principle is that under alkaline conditions, serum transferrin can be bonded to Fe3+, and the remaining unbound Fe3+ can be reduced to Fe2+, the bonded Fe3+ could be released after acidification ,and then be reduced into Fe2+ . A purple compound could be produced by the reaction between Fe2+ and philo oxazine ,and this purple compound has a special wave at 562 nm. Respectively reading the OD562nm values A1 and A2 , and A1 is based on the unbound Fe3+ , A2 is based on the bounded Fe3+ . And the concentration of TIBC is proportionate to the difference between A2 and A1.
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